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  Lipid transporters

The cell interior of Gram-negative bacteria is protected by a double layer of membranes. While the inner one is mainly composed of phospholipids (PLs), the outer membrane is built as an asymmetric bilayer, where the inner part consists mainly of PLs, while the outer membrane contains lipopolysaccharides (LPS). This asymmetry plays an essential part in drug resistance, as the membrane system works as a barrier against all kinds of pharmaceutics (e.g. antibiotics). To maintain the composition of these membranes, there are PL- and LPS-transport systems in bacteria. The molecular principles that control these transport systems are the subject of our investigations - currently we are working on the Lpt- and the Mla system, which have come into focus as potential antibiotic targets. Through native mass spectrometry, in particular LILBID MS, we aim to analyse the interactions between the components of the transport systems. Furthermore, we try to follow the path a lipid or a LPS takes through the systems by binding studies. 
lipid transport