regulation of SRSF protein interactions
Important
aspects of mRNA maturation, such as alternative splicing and quality
control, take place in nuclear speckles in the nucleus. The long
non-coding RNA called MALAT1 is highly relevant for these events, as it
guides the activity of interacting partners such as splicing factors by
binding them in a phosphorylation dependent manner. One of those
splicing factors are SRSF proteins which can be not, half or fully
phosphorylated. These phosphorylation states determine residence times
in the nuclear speckles as well as interactions between the SRSF
proteins, MALAT1, mRNAs and other splicing factors. This dynamic
interplay must be controlled by changes of the binding constants
between the different interaction partners. We are interested in the
molecular principles guiding these processes. We are investigating the
relevant binding-events in a top-down manner, using nuclear speckle
minimal systems containing different SR proteins, a library of MALAT1
sequences, kinases or phosphatases as well as mRNA.
